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Association of circulating levels of miRNA-204 and the presence and severity of coronary artery calcification in patients with type 2 diabetes: results from an observational study
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Authors:Yao-Dong DING1;Yu-Qiang PEI1;Rui WANG1;Jia-Xin YANG1;Ying-Xin ZHAO1;Xiao-Li LIU1;Hua SHEN1;Shuo ZHANG2;Hai-Long GE3

Author Affiliation:1.Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China;2.Department of Gynecology and Obstetrics, Beijing Tiantan Hospital, Capital Medical University, Beijing, China;3.Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China


Abstract: Background The basic studies have indicated that miRNA-204 downregulation might regulate vascular smooth muscle cell calcification in vitro. The purpose of this study was to investigate the association between circulating miRNA-204 and coronary artery calcification (CAC) in patients with type 2 diabetes mellitus (T2DM). Methods In this retrospective study, we consecutively enrolled 179 individuals with T2DM who underwent coronary computed tomography (CT) in Beijing Anzhen Hospital between January 2015 and September 2016. The CAC scores were assessed by the 320-slice CT scanner. Circulating miRNA-204 levels were measured by quantitative real-time polymerase chain reaction. Results Significant CAC (CAC scores > 10 Agatston units for significant calcification) was observed in 98 patients (54.7%). The miRNA-204 levels (relative expression) were significantly lower in patients with significant CAC than non-CAC controls (1.001 ± 0.100 vs. 0.634 ± 0.211, P < 0.001). The miRNA-204 levels were negatively correlated with the glycated hemoglobin A1c (HbA1c) level (r = -0.702, P < 0.001), CAC scores (r = -0.710, P < 0.001), and the United Kingdom Prospective Diabetes Study (UKPDS) score (r = -0.355, P < 0.001). According to multivariate logistic analyses, the miRNA-204 levels were still significantly and independently associated with the presence of CAC after adjustment for the conventional risk factors. Receiver-operating characteristic curve analysis showed that circulating miRNA-204 levels can predict the severity and extent of CAC, and the specificity was higher than that of the traditional risk factors HbA1c and UKPDS. Conclusions The results detected that miRNA-204 downregulation was associated with increased CAC in patients with T2DM. Circulating levels of miRNA-204 may be an independent risk factor for the presence of CAC.


Calcification; Coronary artery disease; MicroRNA-204; Type 2 diabetes mellitus
Received:July 30, 2020        Accepted:August 20, 2020   Published Online:August 27, 2020
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